How long can stem cells be stored?
Who knew that the
umbilical cord blood which was once shrugged off as trash could hold
life-saving stem cells?
Stem cells
follow the same analogy in multicellular organisms as seeds do in plants. They
are undifferentiated biological cells that form the building blocks for
different types of cells and have the ability to produce more of the same type
of cells.
The ability of these
cells to treat around 70 blood-related and genetic disorders has earned it a
coveted reputation lately in the biological world. Scientists have devised
methods for stem cell preservation to last a lifetime. Scientific evidence otherwise suggests that
they can be stored for up to 24 years .
Why preserve stem cells?
Young cells with regeneration ability : The stem cells from the umbilical cord are
juvenile and raw in their exposure to the outside environment, hence the process of baby stem cell preservation is very important. They can differentiate and regenerate into different
types of cells in the body and come in aid during severe disorders.
Treats blood disorders : Cord blood cells hold the power to treat several fatal diseases
like Sickle cell anaemia and thalassemia which almost 15,000 infants in our
country are born with.
Life saver for the entire family : Although there are 13 million volunteer donors
registered globally, the chance to find one for Asians drops to 1 out of 10,
especially amongst Indians since they have a more diverse HLA type.
Future of emerging research : Currently researchers are studying effect of
stem cells on spinal cord, heart and brain injury. These magical cells have
made it possible to question the unfathomable in medical field.
One for the family : Cord blood stem cells are genetically unique. In cases where a
family has a history marked with diseases like Alzheimer’s disease , asthma,
heart disease and stroke, there is a higher chance of them seeping into their
children through inheritance . These diseases can be cured with cord blood.
Stem cell preservation steps :
Step 1: First of all, the cord lining is
collected by cutting off a segment of the umbilical cord of a newborn child .
This enables cord blood to be collected. The clamped segment of the cord is
kept in a sterile container that comes inside the umbilical cord collection
kit.
Step 2: The umbilical cord collected is sent to
Cordlife’s laboratory where the technologist verifies the identity of the right
client and performs further steps to decontaminate the cord in a bio hazard safety cabinet. Then, the cord undergoes processing and cryopreservation.
Step 3: Then the umbilical cord is further processed to
separate all other components through centrifugation to leave only the cord
lining. The cord lining thus obtained is cleaned with wash buffer solution and
segmented into small pieces.
Step 4: The
decontaminated segmented cord linings are then transferred into tiny tubes or
cryovials loaded with cryoprotectant to maintain their viability during
cryopreservation.
Step 5: The cord lining segments are frozen gradually in
the controlled-prate freezer, where the temperature is lowered by 1-2°C per minute.
Step 6: After this, the cryovials are shifted into a MVE
anti-contamination vapour phase liquid nitrogen storage system where the
temperature is maintained at -190°C for a long-term cryopreservation.
Comments
Post a Comment